2017 Volume 74 Issue 6 Pages 517-523
This study presents the influence of the preparation conditions of conducting polymer films on the bioelectrocatalytic currents generated with enzyme electrodes composed from those films and glucose oxidase. The films were prepared by electrochemical polymerization of 3-methylthiophene and thiophene-3-acetic acid. The enzyme electrodes were finalized by covalently immobilizing glucose oxidase on the film surface. The bioelectrocatalytic current was measured in a phosphate buffer solution (pH 7.0) containing glucose and p-benzoquinone. By switching the polymerization solvent from acetone to acetonitrile, the bioelectrocatalytic current increased by a factor of 7.2. The monomer concentration had an optimum range (0.25–1.0 M) for the bioelectrocatalytic current. The polymerization current increased accompanying an increase in applied potential during the electrochemical polymerization. In contrast, the bioelectrocatalytic current showed a plateau in a higher potential range. The primary factor to influence the bioelectrocatalytic current is considered to be the surface morphology of the films that is closely related to the electrochemical polymerization conditions.
