Abstract
Natural nucleobases of DNA exhibit high duplex stability and high orthogonality, which are attractive from chemical viewpoints. If artificial base pairs are developed, it would be possible to develop novel functional materials. We have developed pseudo base pairs by incorporating non-natural molecules into DNA through D-threoninol. Although our pseudo bases are much different from natural nucleobases, they show high duplex stability and high orthogonality similarly to the natural ones. In this paper, we summarize our recent work on pseudo base pairs, focusing on the stability of DNA duplexes containing pseudo base pairs.
