1977 Volume 34 Issue 11 Pages 813-817
An immunoresponsive membrane, which was prepared by immobilizing cardiolipin antigen, was applied to assemble an immunosensor for serological tests for syphilis. The sensor consists of the membrane potential measuring system, in which both surfaces of the immunoresponsive membrane are contacted with electrolyte solutions of the same concentration and an immunochemical reaction take place at one of the surfaces. The sensor exhibited an excellent performance in monitoring specifically the syphilis antibody in serum. The output of the sensor reached a steady state value by dipping 5 min, and increased with the increase in the antibody concentration and temperature. It ws concluded that the potential was generated due to an asymmetric charge distribution of the immunoresponsive membrane phase in association with an immunochemical reaction.
