Abstract
In the previous paper (1), a method of isolation of Myc. lepraemurium from infected tissue by means of trypsin digestion prior to the procedure of Hanks' method (2) was described. It is fact, however, that contamination of the tissue component in the final product obtained can not be avoided even through careful procedure. This method has two additional disadvantages; a small yield of the bacilli and it is a complicated procedure.The present paper reports a new and simple isolation and purification method of Myc. lepraemurium, under sterile conditions, from the subcutaneous lepromata of the rat by using trypsin digestion with sodium desoxycholate treatment. The efficiency of this method was determined by observing Ziehl-Neelsen staining, Ziehl Nile blue staining (3) under a light microscope, and electron micrographes. Some enzymatic activities and the remaining infectivity of the final product as compared to that of the starting material were also observed.
