EFFECTS OF DEXTRAN SULFATE ON PLAQUE FORMATION OF ENTEROVIRUSES

Abstract

Takemoto and Kirschstein (1) found that the attenuated strain of type 1 poliovirus (sabin) contained three different plaque types when assayed with agar overlay medium containing dextran sulfate, and further investigated several biological characteristics of the virus strains with the largest and the smallest plaque types. In the present study, the observations of Takemoto and Kirschstein are repeated on eleven virulent and attenuated strains of polioviruses and seven strains of Coxsackie viruses. Unexpectedly, the enhanced plaque formation as observed by them was not occurred in our experiments. The results, however, shows to be served as a possible tool that discriminate a wild virus strain from live poliovirus vaccine strain concering . at least, with type 1.