GENETIC ANALYSIS OF POLYKARYOCYTOSIS BY HERPES SIMPLEX VIRUS

II. RECOMBINATION BETWEEN VIRUSES WITH NON-FUSING AND FUSING ABILITY

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Mixed infection with non-fusing (S) and fusing (G) viruses produced the recombinant (L) which was non-fusing but formed the plaque intermediate in size between parents. Genetic analysis in combination with IDU-resistance (I^r) demonstrated that the progeny designated as LI^s (IDU-sensitive), LI^r and GI^r were produced at the ratio of 2 : 2 : 1 by genetic recombination between SI^r and GI^s, leading the hypothesis that cell fusion resulting in polykaryocytosis can be expressed by two cistrons each of which determines L character when functioning separately, and recombination units between these L cistrons could be calculated to be 13.0. Further cross experiments using the L-type recombinants suggested that additional cistron (s) would participate in the process involved in cell fusion or that the intracistronic crossovers are possible.