Experimental Study on the Effect of CoQ-10 Administration to Isoproterenol-Induced Cardiomyopathy of Rats

Abstract

It is well-known that administration of isoproterenol (IPA) produces the infarct-like cardiomyopathy to experimental animals. To confirm the biochemical change in energy metabolism of heart mitochondria isolated from rat treated with IPA, the oxygen consumption, lipid peroxide content and CoQ content were determined. The lipid peroxide content was significantly increased in the IPA-treated animals. The respiratory responses of mitochondria indicated the uncoupling of oxidative phosphorylation . The combination use of CoQ-10 with IPA could reduce the lipid peroxidation and respiratory disorders induced by IPA. CoQ homologue level of mitochondria was determined by the method of high pressure liquid chromatography. It was revealed that the deficiency of CoQ homologue was not induced by IPA administration. These studies suggested that lipid peroxidation induced by IPA plays a major role in the development of cardiomyopathy and is prevented by co-administration of CoQ-10.