Abstract
A part of the serial experiments on the preservation of Myc. lepraemurium by means of lyophilization was reported in previous paper (1). The results obtained demonstrated that the infectious activity of Mycobacterium lepraemurium was still evident after two years and five months over of the preservation in vitro by lyophilization, and that this period of maintenance of infectious activity of the bacilli was longer than that of the data reported by Shitama (2) and Lew (3). In the present paper, an additional experiment on the infectious activity of the bacilli which were preserved for five years and five months over in the form of lyoplilization is reported.
