Since a procedure of purification and concentration of virus by means of adsorption to and elution from erythrocytes is a simple and effective method, some experiments of purification of pneumotropic and neurotropic viruses capable of hamegglutination have been made by this method. The studies on purification and concentration of the GD VII strain of mouse encephalomyelitis virus were described by Lahelle and Ward, (1) and Nakagawa and Nakamura (2) (3) in recent years. However, thatt the concentration of the virus by this procedure did not produce satisfactory yields of virus because of hemolysis appearing in the process of concentration of the virus by elution from erythrocytes, and that the combination of the methods by means of methanol and acetone precipitation at low temperature with the method of hemagglutination was attempted for obtaining more satisfactory results, were reported in previous papers (2) (3). The purpose of the present paper is to report the research on further more effective method by means of adsorption to and elution from the red cells stroma (4) which are capable of adsorption of viruses.

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A part of the serial experiments on the preservation of Myc. lepraemurium by means of lyophilization was reported in previous paper (1). The results obtained demonstrated that the infectious activity of Mycobacterium lepraemurium was still evident after two years and five months over of the preservation in vitro by lyophilization, and that this period of maintenance of infectious activity of the bacilli was longer than that of the data reported by Shitama (2) and Lew (3). In the present paper, an additional experiment on the infectious activity of the bacilli which were preserved for five years and five months over in the form of lyoplilization is reported.

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1. A pedicled flap valve prosthesis has been devised using polyethylene for its material.2. This artificial valve was inserted through the left ventricle and placed directly above the aortic valve. It caused no clot formation and the dogs remained alive for a long time.3. Used in valvular insufficiency, the artificial valve abolished the regurgitation very effectively and continued to work as efficient prosthesis over a long period of time.4. This pedicled flap valve prosthesis of our device has a few unsettled problems involved in it ... the unsatisfactory quality of the synthetic resin used, among the rest.(In conclusion, we express our sincere indebtedness to Prof. Hiroshi Miyake who guided us throughout this work and took the trouble of reading this paper in manuscript)

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Many explorations on the deposition of calcium salt have been made in order to make clear the calculus formation, but non of them are yet satisfactory . For explanation of the formation of the calculus, there are two concepts. The one has connected to organic substances in calculus, the other to inorganic substances. The purpose of our study was to attempt to define the mechanism of the formation of solid substances, .\urinary calculus (1), bile concrement, salivary calculus (2), egg shell, blood coagulation, labreaction, gastric calculus, further igneous rocks (3), and snow crystallf orm (4).It was generally accepted that at the early stage of calculus formation in urinary canal, the thin calcium salt called “Ca-plaque” was formed on the surface of mucosus membrane of kidney pelvis, where erosion was observed (5).In the previous report from this laboratory, the electric potential of mucosus membrane was measured after injuring (1). When the membrane surface of horse kidney was slightly sliced, the electric potential became high. By immersing the electrode in urin, the artificial calculus was formed around the positive electrode (1).The present experiment is an effort to contribute something for the explanation of the mechanism of the calculus formation.

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According to, the idea of tissue therapy originated by Filatov we have made the Placenta-Plasma from the cold-stored placenta as a regeneration-promoting agent . Up to the present stage, two preparations of cold-stored human placenta are found uself ul in a wide range of diseases. In the present report, the methods to prepare the above-mentioned preparations are described and the chemical nature of these preparations are shown.

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In 1953 the author observed with a photoelectrical method a constant vibration of normal human hand.Rohracher (1955) found by using a highly efficient pickup that the body surface of warm-blooded animals is vibrating constantly and that this phenomenon cannot be observed in cold-blooded anima's. Since this specific vibration in warm-blooded animals was enhanced in the fevered condition and inhibited in hibernation. Rohracher supposed that it might be caused by the action of the autonomic nervous system which controls body temperature. On account of the finest amplitude of the vibration this phenomenon is called “microvibration” (MV). The present investigationn was undertaken to elucidate the meaning of MV and its originating mechanism more precisely.

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