Evaluation of First-and Second-Generation Assays for Detection of Antibody to Hepatitis C Virus in non-A, non-B Chronic Liver Diseases Evaluation of 1st and 2nd-Generation Assays in NANBH

SHIGERU TOKESHI; MICHIO SATA; TERUKO HINO; TATSUYA IDE; SEIJI NOGUCHI; HIROSHI SUZUKI; HARUO MURAOKA; HITOSHI NAKANO; KYUICHI TANIKAWA

doi:10.2739/kurumemedj.40.27

Evaluation of First-and Second-Generation Assays for Detection of Antibody to Hepatitis C Virus in non-A, non-B Chronic Liver Diseases

Evaluation of 1st and 2nd-Generation Assays in NANBH

SHIGERU TOKESHI, MICHIO SATA, TERUKO HINO, TATSUYA IDE, SEIJI NOGUCHI, HIROSHI SUZUKI, HARUO MURAOKA, HITOSHI NAKANO, KYUICHI TANIKAWA

Author information

SHIGERU TOKESHI
Department of Medicine, Kurume University School of Medicine
MICHIO SATA
Department of Medicine, Kurume University School of Medicine
TERUKO HINO
Department of Medicine, Kurume University School of Medicine
TATSUYA IDE
Department of Medicine, Kurume University School of Medicine
SEIJI NOGUCHI
Department of Medicine, Kurume University School of Medicine
HIROSHI SUZUKI
Department of Medicine, Kurume University School of Medicine
HARUO MURAOKA
Department of Medicine, Kurume University School of Medicine
HITOSHI NAKANO
Department of Medicine, Kurume University School of Medicine
KYUICHI TANIKAWA
Department of Medicine, Kurume University School of Medicine

Keywords: C100-3 assay, KCL-163 assay, 2nd-generation assay, chronic hepatitis, liver cirrhosis, hepatocellular carcinoma

JOURNAL FREE ACCESS

1993 Volume 40 Issue 1 Pages 27-32

DOI https://doi.org/10.2739/kurumemedj.40.27

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Abstract

The positive rates of the second-generation enzyme-linked immunoassay (2nd-generation assay), and two first-generation immunoassays (C100-3 and KCL-163 assay) to test for anti-HCV antibodies in the serum of patients with non-A, non-B chronic liver disease were determined. The clinical usefulness of these assays was also evaluated. The group positive for the 2nd-generation assay alone was compared with that positive for the 2nd-generation and C100-3 assays with respect to the serum GPT levels determined simultaneously with the antibodies. The latter group showed slightly higher GPT levels. These findings suggest that the 2nd-generation assay is useful for the diagnosis of hepatitis C, and that C100-3 and KCL-163 assays are useful indicators of the activity of hepatic disorders.

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