A strain of cucumber mosaic virus (CMV) was isolated from eggplants showing necrotic symptoms on fruits and mosaic on leaves, and the virus was confirmed to be a causal agent of these symptoms. The virus had characteristics as follows: 1) large necrotic lesions and veinal necrosis on inoculated leaves with systemic symptoms either necrosis or mosaic on Vigna sinensis and Phaseolus vulgaris. 2) systemic infection on radish and Chinese cabbage, etc. without any symptoms. 3) spherical particles about 30 nm in diameter. 4) positive reaction to CMV-Y antiserum in agar-diffusion test. 5) thermal inactivation point about 65 C. From these results, it was concluded that the virus was one of the strains of CMV systemically infectious to leguminous and cruci-ferous plants.