研究論文
マウス線維芽細胞の方向転換遊走時における定性的4ステップの体系化
キーワード:
Cell migration,
Veering migration,
Actin stress fibers,
Phase contrast microscopy,
Laser confocal microscopy
2016 年 28 巻 2 号 p. 41-47
詳細
2016 年 28 巻 2 号 p. 41-47
Cell migration is believed to be a cyclic process of protrusion of the leading edge, adhesion to the substrate, and generation of traction to move the cell body forward. Actin stress fiber is one of the cytoskeletal structures inside the cell and it is known to be an important protein complex involved in this process. In this study, long-term observation of cell migration was performed by phase contrast microscopy, and we observed veering migration of the cell, i.e., change in direction during cell migration. Based on the time sequence of the cell outline and the trajectory of the centroid of the cell obtained from the time-lapse phase contrast images, mechanism of veering migration could be schematized into 4 steps in which expansion of the cell membrane perpendicular to the direction of migration was included. Such expansion step was not observed during directional migration. In order to understand the underlying molecular mechanism of veering migration, actin stress fibers were observed by laser confocal microscopy and alignment of stress fibers inside the cell in each schematized step of veering migration was discussed.
