Abstract
New multiphoton microscopy using CARS (Coherent Anti-Stokes Raman Scattering) spectroscopy is described. In CARS microscopy, the image of molecular vibration that is sensitive to the molecular spices and conformation is obtainable without staining with three-dimensional resolution of sub-micrometer. In this article, optical property of CARS microscopy by the diffraction theory and the developed system using a picosecond tunable laser are described. The multi-focus excitation using a rotating-microlens array enables high speed spectral imaging.
