Abstract
This work reports the measurement of the transverse distribution of UV laser-induced fluorescence in peanut (Arachis hypogaea L.) leaves by an ISA (Imaging Spectrogram Analysis) system. The ISA system comprised a microscope, an imaging spectrograph, a chilled-CCD camera and image measurement devices. The fluorescence spectra on any line in a section of a leaf were examined in detail. Results reveal that the chlorophyll fluorescence ratio (F740/F685) increased according to the depth from the surface in the control leaf, while it maintained a constant value in the UV-B-exposed leaf. Blue fluorescence (400 nm-500 nm) increased remarkably under UV-B irradiation in the upper epidermis of the leaf. It is thought that this method is useful for examining the growth conditions of plant leaves.
