抄録
In order to evaluate Photodynamic Therapy (PDT) internal energy kinetics with pulsed excitation, we constructed an experimental setup combining time gate and spectroscopic separation factors in order to obtain the time-resolved phosphorescence waveform from the clinical photosensitizers at the spectral peak. We employed a fast optical shutter as the time gate, and a monochrometer as the spectroscopic separation factor to cut off high intensity fluorescence. We also verified the lifetime and spectrum of the measured luminescence. We subsequently concluded that we had successfully obtained the time-resolved phosphorescence waveform from Talaporfin sodium (NPe6). We believe that the behavior of the photosensitizer fluorescence and the singlet oxygen fluorescence are within reason from the point of the view of the energy flow as determined in PDT kinetics.
