抄録
The control of the site of the gene transfection in the body is strongly desired to achieve effective and safe gene therapy. For this purpose, we have recently developed novel light-responsive gene carriers (ternary complexes), in which the pDNA/polycation polyplex is enveloped with the anionic dendritic photosensitizers for effective light-induced transfection. The ternary complexes achieved more than a 100-fold photochemical enhancement of the transgene expression in vitro with minimal photocytotoxicity. The subconjunctival injection of the ternary complexes in rat eyes followed by the laser irradiation resulted in an appreciable gene expression only at the laser-irradiated site. These light-responsive gene carriers are expected to be useful for the site-directed transfection in vivo.
