巻号一覧
35 巻, 7 号
選択された号の論文の8件中1～8を表示しています
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佐藤 俊一2007 年 35 巻 7 号 p. 428-429
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーPDF形式でダウンロード (236K) -
細川 陽一郎, 安國 良平, 梶 貴博, 山口 敦, 井口 世利也, 朝日 剛, 増原 宏2007 年 35 巻 7 号 p. 430-435
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーWhen a near-infrared (NIR) femtosecond laser is focused in water, several kinds of nonlinear phenomena, such as shockwave propagation, cavitation bubble generation, and jet flow, are induced in the vicinity of the laser focal point. Such phenomena are also induced by near-infrared nanosecond and picosecond lasers, but femtosecond irradiation allows a better localization. Moreover NIR femtosecond laser focussing permits to perform a local processing of cell and tissue because of its efficient multi-photon absorption and photo-mechanical ablation mechanism. In this study, the impulsive force generated in cell culture medium by these nonlinear phenomena was applied to individual control of single animal cells. Mouse NIH-3T3 fibroblast cells cultured on a substrate were independently isolated by the impulsive force, and patterned by transferring them to another substrate. Furthermore nanoparticles and biological molecules are transfected to the cell by the local processing of the cell membrane. These demonstrations are promising for future femtosecond laser methodology for bioscience and biotechnology.抄録全体を表示PDF形式でダウンロード (17926K) -
須丸 公雄, 金森 敏幸2007 年 35 巻 7 号 p. 436-440
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーIn order to meet the diversifying demand for the cell manipulation in the rapid progress of cell engineering, we developed a novel technique to control living adherent cells on a culture substrate by irradiating light onto multiple points simultaneously. In clear contrast to the conventional cell patterning using the previously patterned substrate, the cell-retaining area can be defined even after cell seeding, and the captured cells can continue to grow freely beyond the defined area afterwards. Also the patterned co-culture of living cell was demonstrated by introducing living cells sequentially onto patterned cell adhesion areas assigned and created in each step on a newly developed photo-responsive culture substrate.抄録全体を表示PDF形式でダウンロード (12002K) -
西山 伸宏, 片岡 一則2007 年 35 巻 7 号 p. 441-444
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーThe control of the site of the gene transfection in the body is strongly desired to achieve effective and safe gene therapy. For this purpose, we have recently developed novel light-responsive gene carriers (ternary complexes), in which the pDNA/polycation polyplex is enveloped with the anionic dendritic photosensitizers for effective light-induced transfection. The ternary complexes achieved more than a 100-fold photochemical enhancement of the transgene expression in vitro with minimal photocytotoxicity. The subconjunctival injection of the ternary complexes in rat eyes followed by the laser irradiation resulted in an appreciable gene expression only at the laser-irradiated site. These light-responsive gene carriers are expected to be useful for the site-directed transfection in vivo.抄録全体を表示PDF形式でダウンロード (4547K) -
佐藤 俊一, 寺川 光洋, 小原 實2007 年 35 巻 7 号 p. 445-447
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーIn vivo site-selective gene transfection by the use of laser-induced stress wave (LISW) is described. In this method, targeted tissue is injected with plasmid DNA, on which a light-absorbing material is placed as a laser target. The target is irradiated with high-power, nanosecond laser pulse to produce plasma; its expansion is accompanied by high-peak-pressure, compressive pressure wave which is called LISW. The interaction of cells with LISW (s) can induce gene transfection in the targeted tissue. On the basis of this method, highly siteselective transfection in rat skins and mouse brains has been demonstrated in vivo. Application of this method to tissue engineering, i.e., the development of high-performance skin grafts, is also reported.抄録全体を表示PDF形式でダウンロード (8211K) -
渡辺 歴, 松永 幸大, 福井 希一, 伊東 一良2007 年 35 巻 7 号 p. 448-452
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーFemtosecond laser pulses in the near-infrared region can be used to selectively disrupt and dissect intracellular organelles. We review intracellular surgery of organelles in living cells using a femtosecond laser. We demonstrate surgery of mitochondria in living HeLa cells in vitro using an amplifier with a repetition rate of 1 kHz and only a femtosecond laser oscillator with a repetition rate of 76 MHz. The viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser can be performed without compromising the longterm cell viability. Femtosecond laser-based nanosurgery has the possibility to provide space-selective information on the function and dynamics of organelles in living cells.抄録全体を表示PDF形式でダウンロード (8593K) -
片岡 洋祐2007 年 35 巻 7 号 p. 453-456
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーLow-reactive level laser therapy and photo-dynamic therapy have become popular in many clinical fields. Herein, the possible application of these techniques to the central nervous system is discussed. Low-reactive level laser irradiation with a wavelength of 830 nm has been shown to suppress neuronal activities in the rodent brain and increase tissue ATP levels. Such effects of lasers may facilitate the protection of neurons from ischemic brain insults. In addition to these techniques, we have demonstrated that photo-oxidation of the cerebral cortex by photo-irradiation following the injection of photosensitizing dye induces spreading depression, the propagation of neuronal membrane depolarization throughout the cortical hemisphere. Spreading depression potently induced the proliferation of progenitor cells, which have multipotency in cellular differentiation. Thus, this photo-dynamic technique may facilitate the regeneration of brain tissue. We believe that these phototherapeutic techniques will usher in a new frontier in the medical science of the central nervous system.抄録全体を表示PDF形式でダウンロード (4902K) -
植野 能史, スマン ゲオルグ, 住谷 明, 遠藤 彰2007 年 35 巻 7 号 p. 457-461
発行日: 2007/07/15
公開日: 2014/03/26
ジャーナル フリーWe have characterized CO2 laser generated debris from plane tin (Sn) targets with gold coated quartz crystal microbalances (QCM). Sputtering was the dominant process for 1mm and 15μm thick solid Sn targets. In addition, average conversion efficiency at 13.5nm of 2.0% within 2% full bandwidth and 2π sr solid angle was obtained. This indicates that a high CE without Sn debris deposition on a multi-layer collector mirror can be obtained.抄録全体を表示PDF形式でダウンロード (4490K)
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