表面プラズモン顕微鏡による細胞接着部位の無標識観察

抄録

Observation of cell adhesion site without chemical labeling contributes to the understanding of intracellular adhesion dynamics of biomolecules including integrin and other adhesion proteins. To satisfy the demands to investigate cell adhesion site without labeling, we employed a scanning localized surface plasmon microscopy (SLSPM) and observed the adhesion sites of the cell on a substrate with spatial resolution of 200 nm. By combining a non-scanning surface plasmon microscopy (SPM) and SLSPM, we observed a cell adhesion site and identifi ed the regions of interest. The main advantage of our system is that it has the highest spatial resolution among other SPM imaging systems, because size of an electric field distribution, which determines the spatial resolution, can be minimized by interference of surface plasmons excited with radially polarized light. In addition, we discuss the objectives to use the SLSPM and SPM systems reported by other groups for cell observation.