抄録
Neurons perform fast information processing in the form of membrane potential dynamics. Therefore,
detailed examinations of membrane potential dynamics in neurons are crucial to the understanding of
neuronal information processing and brain functions. However, attempts to characterize membrane
potential dynamics in neurons have been hampered by the small size and the complexity of neurites,
such as dendritic spines and axons, where conventional electrophysiological techniques have limited
access. Furthermore, while optical imaging techniques provide better access to these structures,
fluorescence-based imaging techniques are not suitable for quantitative membrane potential
measurements. Second harmonic generation (SHG) imaging, which can overcome these problems, can
be utilized for quantitative membrane potential imaging. Here, I review the basic principles and
applications of SHG imaging in neuroscience researches and discuss the potential of this technique.
