We measured the cerebral blood flow (CBF) of mice by laser speckle flowgraphy (LSFG) and laser Doppler fl owmetry(LDF). In LSFG, two parameters that indicate CBF, mean blur rate (MBR) and the reciprocal of correlation time (CT), are calculated from speckle patterns. MBR is calculated from 2 × 2 pixels in successive three frames, but CT is calculated from 7 × 7 pixels in one frame. The CBF change is caused by the brain activation evoked by whisker stimulation, and the MBR and CT time courses measured by LSFG were compared with the time course by LDF to validate the temporal resolution of LSFG. Both MBR and CT time courses were highly correlated with the LDF results. The spatial resolution of the MBR map is higher than that of the CT map and MBR is less infl uenced by the noise.
