顕微ラマン分光法による神経細胞成熟のモニタリング分析

抄録

We applied Raman spectroscopy to monitor the molecular compositional changes during the maturation of cultured neural cells. Since the Raman spectra of the nucleus and cytoplasm of neural cells are slightly different, we employed the Raman spectra of the nucleus for further analysis. We analyzed with PLS regression the spectra of live neural cells measured after 2, 8, 15, and 60 days of culturing. Our results suggest that functional progress and maturation are related with molecular compositional changes in the cell and demonstrate the high potential of Raman spectroscopy in monitoring functions of live neural cells without labeling.