抄録
Optical microscopes are effective tools for cellular function analysis because biological cells can be
observed non-destructively and non-invasively in the living state in either water or atmosphere condition.
Label-free optical imaging technique such as phase-contrast microscopy has been analyzed many
cellular functions, and it is essential technology for bioscience field. However, the diffraction limit of
light makes it is difficult to image nanostructures in a label-free living cell, for example the endoplasmic
reticulum, the Golgi body and the localization of proteins. Here we demonstrate the dynamic imaging of
a label-free cell with high spatial resolution by using an electron beam Excitation-Assisted optical (EXA)
microscope. We observed the dynamic movement of the nucleus and nanoscale granules in living cells
with better than 100 nm spatial resolution. Our results contribute to the development of cellular function
analysis and open up new bioscience applications.
