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46 巻, 9 号
「ナノ・マイクロオプティクスを利用したバイオ・ケミカルセンシング応用」特集号
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「ナノ・マイクロオプティクスを利用したバイオ・ケミカルセンシング応用」特集号
特 集
レーザー解説
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西山 道子2018 年 46 巻 9 号 p. 488-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスRecently, nano-optics focusing on near-field light and micro-optics based on elements size of tens of to a few hundred micrometers have been utilized to biochemical sensing application because they can improve the sensitivity, responsibility and detection limit. This special issue will introduce innovative researches about biological and chemical sensing application based on nano-micro optics.抄録全体を表示PDF形式でダウンロード (227K) -
三宮 工2018 年 46 巻 9 号 p. 490-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスMetallic nanostructures exhibit optical resonance, where free electrons oscillate at the surface, creating strong near field. This resonance wavelength is sensitive to the surrounding refractive index, and therefore a subtle change of the surrounding environment can be monitored by tracking the resonance. This sensing scheme can be applied to detect biological targets by properly functionalizing the surface. In this article we describe how such nano-biosensors work as well as application examples using plasmonic nanostructures. Since the optical fi eld distribution at the metal surface determines the sensor performance, we also show how nanoscopic optical fi eld can be experimentally visualized by electron microscopy based methods.抄録全体を表示PDF形式でダウンロード (1023K) -
白石 正彦, 窪寺 昌一2018 年 46 巻 9 号 p. 495-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスA novel spectroscopic measurement method has been established that enables to detect biomolecules in a simple measurement configuration with a small absolute amount of sensing targets. A spectroscopic cell with a sensing volume of sub-picoliter has been fabricated inside a glass optical fiber by use of near ultraviolet (NUV) femtosecond laser pulses. By use of the cell with a combination of a halogen white light source and a compact CCD spectrometer, spectroscopic measurements using localized surface plasmon resonance (LSPR) were demonstrated. Absorption spectra using LSPR, which had the absorption peak centered at 518 nm, were obtained when a solution of gold nanoparticles (GNPs) with a diameter of 5‒10 nm was injected into the cell with a volume of 0.4 pL. An aggregation of GNPs caused by biomolecules of L-cysteine was monitored by the change in the absorption spectra. The absorption peak of LSPR decreased due to an injection of 7.5 mM of L-cysteine with the detection sensitivity of 3.0 × 10‒15 mol (3.6 × 10‒13 g).抄録全体を表示PDF形式でダウンロード (599K) -
川田 善正, 居波 渉2018 年 46 巻 9 号 p. 500-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスOptical microscopes are effective tools for cellular function analysis because biological cells can be observed non-destructively and non-invasively in the living state in either water or atmosphere condition. Label-free optical imaging technique such as phase-contrast microscopy has been analyzed many cellular functions, and it is essential technology for bioscience field. However, the diffraction limit of light makes it is difficult to image nanostructures in a label-free living cell, for example the endoplasmic reticulum, the Golgi body and the localization of proteins. Here we demonstrate the dynamic imaging of a label-free cell with high spatial resolution by using an electron beam Excitation-Assisted optical (EXA) microscope. We observed the dynamic movement of the nucleus and nanoscale granules in living cells with better than 100 nm spatial resolution. Our results contribute to the development of cellular function analysis and open up new bioscience applications.抄録全体を表示PDF形式でダウンロード (2391K) -
三林 浩二2018 年 46 巻 9 号 p. 506-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスBiofluorometric bio-sniffers (biochemical gas sensor) for acetone and isopropanol (IPA) were constructed by connecting a UV-LED and a photomultiplier tube to an optical fiber with a flow-cell equipped an S-ADH (secondary alcohol dehydrogenase) membrane. Gaseous acetone and IPA were measured by detecting auto-fluorescence of nicotinamide adenine dinucleotide (NADH) (ex. 340 nm, fl. 491 nm) as a coenzyme of S-ADH redox reactions. Both breath volatiles from healthy and diabetic subjects were analyzed by the bio-sniffers, thus diabetic acetone showing significant higher than that of heathy subjects. Similarly, a volatile-imaging system (sniff-cam) employing alcohol dehydrogenase (ADH) that detects acetaldehyde (AcH) vapor was also developed using a ADH mesh, a UV-LED array sheet and high sensitive camera, and applied for imaging AcH in breath and skin gas after drinking. The ADH sniff-cam allowed to visualize the concentration distribution of AcH in breath and skin gas. The biofluorometric sniff-devices would be useful for non-invasive disease screening and assessment.抄録全体を表示PDF形式でダウンロード (1279K) -
細木 藍, 西山 道子, 井川 寛隆, 渡辺 一弘2018 年 46 巻 9 号 p. 512-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスWe developed a novel fiber optic Surface Plasmon Resonance (SPR) hydrogen sensor based on heterocore structured fiber optics with multi-layer films of gold (Au), tantalum pentaoxide (Ta2O5), and palladium (Pd) that are uniformly coated on a cladding surface. In a light intensity-based experiment with Light Emitting Diode (LED) operation at 850 nm, we induced a transmitted loss change of approximately 0.23 dB with a response time of 15 s for 4% hydrogen in 25-nm Au, 60-nm Ta2O5, and 3-nm Pd multi-layer films. In addition, multi-point detection for hydrogen was successfully achieved using a combination of an SPR hydrogen tip sensor and an interrogating system based on Pseudorandom Noise-code Correlation Reflectometry (PNCR). The sensor gives optical loss changes with and without hydrogen absorption and shows Pd hydrogenation process in real time with high sensitivity, such as more than 0.4 dB.抄録全体を表示PDF形式でダウンロード (1001K) -
橋本 修一2018 年 46 巻 9 号 p. 517-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスPlasmonic nanoparticles have emerged as unique nanoheaters that selectively heat the local environment of the particles. This local heating finds various applications - from photothermal cancer therapy and photothermal imaging to photothermally-enhanced catalytic activity and photothermal thermoelectric devices. This review deals with the interactions of lasers with plasmonic nanoparticles - from fundamental aspects of plasmonic heating to practical applications such as drug delivery, phase separation of thermoresponsive polymers, hydrothermal reactions, and glass nanoprocessing, to name a few. Plasmonic heating helps to reveal fundamental physics such as phase transition and phase separation at the nanoscale. Besides, plasmonic heating is valuable for microscale fabrication and manipulation. Further, plasmonics incorporating photothermal effects can be a powerful technique for temperature sensing.抄録全体を表示PDF形式でダウンロード (1439K)
一般論文
レーザーオリジナル
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冨田 孝幸, 西澤 直人, 宇都宮 成弥, 齊藤 保典2018 年 46 巻 9 号 p. 522-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスA Laser Induced Fluorescence Spectrum (LIFS) is useful for object identification, and LIDAR is powerful in the remote distribution monitoring of objects. We developed a LIFS-LIDAR system whose characteristics allow it to be a potential apparatus for environmental observations. Our system consists of a UV (355 nm) pulse laser, a 25-cm diameter telescope on an altazimuth mount, and a multi-channel spectrometer equipped with a gated image-intensified CCD. To observe low LIFS signal objects even during the daytime, the CCD’s gate-open time width was set to 10 ns to reduce the background light noise. The gating was delayed to coincide with the fluorescence arrival time to the CCD, which provided information on the object distribution. System performances were checked by the monitoring fluorescence spectra of a living plant and a wooden board inside a room under a natural light condition. The plant had fluorescence peaks at around 420, 680, and 740 nm; however, the wooden board only had a peak at around 420 nm, showing that LIFS is a good indicator for object identification. Our system’s volume, 1280 × 830 × 990 mm, was so compact that it was easily carried by a compact vehicle; we also prepared a small dynamo. Our mobile LIFS LIDAR will allow observations more freely any time.抄録全体を表示PDF形式でダウンロード (701K) -
Hidetoshi NOJIRI, Masayuki OKOSHI2018 年 46 巻 9 号 p. 527-
発行日: 2018年
公開日: 2020/12/18
ジャーナル オープンアクセスSilicone-coated polycarbonate was photochemically modified into SiO2 by a 157-nm fluorine laser for developing an automotive resin window to satisfy United Nations Economic Commission for Europe (UNECE) regulations for the abrasion resistance of ΔH1000≦2%. We considered a mesh mask. With it, we improved the abrasion resistance by increasing the laser irradiation time to less than 30 s; by increasing the laser irradiation, the abrasion resistance decreased. We revealed that abrasion resistance can be suppressed by lowering the opening length of the mesh mask using laser irradiation through it. By suppressing the stress caused by the photochemical modification by setting the distance between the mesh mask and the sample surface, good abrasion resistance less than 2% was obtained for any laser irradiation time at 20 mm.抄録全体を表示PDF形式でダウンロード (972K)
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