抄録
Recent advances in optics and laser technology have led to the development of visualization and analysis
methods for cellular physiological functions. Two-photon microscopy, which is indispensable due to its
minimal invasion of living specimens, is laser scanning fluorescence microscopy that utilizes two-photon
excitation processes induced by near-infrared ultrashort laser pulses. We have been promoting the use of
semiconductor lasers, adaptive optics, vector beams, and nanomaterials to improve the spatial resolution
or the observation depth. We successfully visualized the activity of hippocampal CA1 neurons in the
deep brain of mice at video rates without resecting the neocortex. We also proposed using fluoropolymer
nanosheets for in vivo imaging and improved the spatial resolution by optical technologies based on
adaptive optics.
