Mammal Study
Online ISSN : 1348-6160
Print ISSN : 1343-4152
ISSN-L : 1343-4152
Original papers
A new pair of primers for molecular sexing of the Pallas squirrel, Callosciurus erythraeus, and variation in fetal sex ratio
Miki KusaharaYoshitaka KamimuraNoriko TamuraFumio Hayashi
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2006 年 31 巻 2 号 p. 87-92

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The Pallas squirrel, Callosciurus erythraeus, is an introduced species to Japan where a male-biased sex ratio has been reported at the weaning stage in the field. For further examination of their earlier (fetal) sex ratio, a new PCR primer set was designed to amplify a portion of the Sry (sex-determining region Y) gene. This successfully produced sex-specific PCR profiles for known-sex adult samples. Using this technique we could determine the sex of fetal samples dissected out of mothers shot as pests on Izu-Oshima Island, Tokyo, Japan. The fetal sex ratio was significantly male-biased in 2000, but not in other years. The sex ratio tending towards male-bias in this squirrel is discussed from a local resource competition theory.
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© 2006 The Mammalogical Society of Japan
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