Japanese Journal of Microbiology
Print ISSN : 0021-5139
Studies on Delayed Hypersensitivity of Antigens Isolated from Mycoplasma pneumoniae Cells
Masuko SUZUKIYoshiro HAYASHISumio ARAIKatsuo KUMAGAI
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1976 年 20 巻 3 号 p. 191-196

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Cells of Mycoplasma pneumoniae Mac strain were fractionated into acetone-soluble and insoluble fractions. Acetone-insoluble fractions were digested with pronase and further purified by chromatography on Sephadex G-75, yielding three water-soluble fractions which were free from lipid and consisted mainly of polysaccharide-protein complex. All these water-soluble fractions possessed eliciting antigenicity to delayed hypersensitivity for M. pneumoniae as measured by skin reactions and macrophage migration inhibition tests, but not to complement-fixing antibodies. In contrast, the acetone-soluble fraction was reactive with the complement-fixing antibodies but not for the delayed hypersensitivity.
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