Method of Genomic DNA Cloning by the Combination of Cosmid Shuttle Vector and Monoclonal Antibody

Masatoshi TAGAWA; Toshihiro ITO; Masamoto KANNO; Akira OKITSU; Tohru SAKAMOTO; Kenji IMAI; Ichiro KUWABARA; Haruhiko KOSEKI; Hisahiro MATSUBARA; Yun-Fai LAU; Masaru TANIGUCHI

Masatoshi TAGAWA, Toshihiro ITO, Masamoto KANNO, Akira OKITSU, Tohru SAKAMOTO, Kenji IMAI, Ichiro KUWABARA, Haruhiko KOSEKI, Hisahiro MATSUBARA, Yun-Fai LAU, Masaru TANIGUCHI

著者情報

Masatoshi TAGAWA
Department of Immunology, School of Medicine, Chiba University
Toshihiro ITO
Department of Immunology, School of Medicine, Chiba University
Masamoto KANNO
Department of Immunology, School of Medicine, Chiba University
Akira OKITSU
Department of Immunology, School of Medicine, Chiba University
Tohru SAKAMOTO
Department of Immunology, School of Medicine, Chiba University
Kenji IMAI
Department of Immunology, School of Medicine, Chiba University
Ichiro KUWABARA
Department of Immunology, School of Medicine, Chiba University
Haruhiko KOSEKI
Department of Immunology, School of Medicine, Chiba University
Hisahiro MATSUBARA
Department of Immunology, School of Medicine, Chiba University
Yun-Fai LAU
Howard Hughes Medical Institute, and Department of Physiology and Medicine, University of California
Masaru TANIGUCHI
Department of Immunology, School of Medicine, Chiba University

ジャーナルフリー

1988 年 32 巻 10 号 p. 1073-1078

詳細

抄録

We report here the strategy to isolate the DNA fragment of any species origin which encodes cell surface antigen by using cosmid library transfection and cell sortings with a monoclonal antibody. We took the mouse melanoma antigen defined by monoclonal antibody as a model system and rescued the genomic DNA by in vitro packaging, showing the feasibility of this procedure.

責任著者(Corresponding author)

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