抄録
It has been known that RNA has a lot of modified nucleosides which are post-transcriptionally introduced by specific enzymes. Modified nucleosides play an important role in the RNA function. In the case of transfer RNA, modified uridine at the first letter of anticodon is thought to participate in the selection of cognate codon so as to ensure the accurate translation. In this report, we tried to detect unknown species of modified uridine from bovine mitochondrial tRNAs by ESI/iontrap mass spectrometer with a new method for derivatizing the modified uridines with CMC [N-cyclohexyl-N′-b-(4-methylmorpholinium)ethylcarbodiimide].
