Mass Spectrometry
Online ISSN : 2186-5116
Print ISSN : 2187-137X
ISSN-L : 2186-5116
Technical Report
An Alternative Method for Quantitative Mass Spectrometry Imaging (q-MSI) of Dopamine Utilizing Fragments of Animal Tissue
Erika NaganoKazuki OdakeShuichi Shimma
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Supplementary material

2023 Volume 12 Issue 1 Pages A0128

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Abstract

Mass spectrometry imaging (MSI) is a well-known method for the ionization of molecules on tissue sections and the visualization of their localization. Recently, different sample preparation methods and new instruments have been used for MSI, and different molecules are becoming visible. On the other hand, although several quantification methods (q-MSI) have been proposed, there is still room for the development of a simplified procedure. Here, we have attempted to develop a reproducible and reliable quantification method using a calibration curve prepared from tissue debris of a frozen section of a sample when we trim the frozen blocks. We discuss the reproducibility of this method across different sample lots and the effect of the biological matrix (ion suppression) on our results. The quantitative performance was evaluated in terms of accuracy and relative standard deviation, and the reliability of the quantitative values obtained by matrix-assisted laser desorption/ionization-MSI was further evaluated by enzyme-linked immunosorbent assay (ELISA). Our q-MSI method for the quantification of dopamine in mouse brain tissue was found to be highly linear, accurate, and precise. The quantitative values obtained by MSI were found to be highly comparable (>85% similarity) to the results obtained by ELISA from the same tissue extracts.

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© 2023 Erika Nagano, Kazuki Odake, and Shuichi Shimma

This article is licensed under a Creative Commons [Attribution-NonCommercial 4.0 International] license.
https://creativecommons.org/licenses/by-nc/4.0/
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