Mass Spectrometry
Online ISSN : 2186-5116
Print ISSN : 2187-137X
Original Article
Quantification of Small Molecule Drugs in Biological Tissue Sections by Imaging Mass Spectrometry Using Surrogate Tissue-Based Calibration Standards
Nozomi TakaiYukari TanakaHideo Saji
Author information
JOURNALS FREE ACCESS FULL-TEXT HTML

Volume 3 (2014) Issue 1 Pages A0025

Details
Full Text-HTML Download PDF (3597K) Contact us
Abstract

Quantitative analysis of administered drugs in biological tissues is essential for understanding the mechanisms underlying their efficacy or toxicity. Imaging mass spectrometry (IMS) may allow the quantification of targeted drugs in tissue sections along with the visualization of their spatial distribution. In this study, surrogate tissue-based calibration standards were prepared to quantify a small molecule drug (S-777469 or raclopride) in tissue sections of mice administered with the drug, followed by analysis with a linear ion trap mass spectrometer equipped with a matrix-assisted laser desorption/ionization (MALDI) source. The distribution of the drugs in the dissected organs was clearly visualized by MALDI-IMS. The drug concentration determined using the calibration standards prepared for MALDI-IMS analysis was highly consistent with that determined by liquid chromatography-tandem mass spectrometry, and the quantification in multiple organs was enabled. The results of this study show that MALDI-IMS can be used to quantify small molecule drugs in biological tissue sections using surrogate tissue-based calibration standards.

Information related to the author
© 2014 The Mass Spectrometry Society of Japan
Next article

Recently visited articles
feedback
Top