Mass Spectrometry
Online ISSN : 2186-5116
Print ISSN : 2187-137X
Original Article
High-Throughput Analysis of Sucrose Fatty Acid Esters by Supercritical Fluid Chromatography/Tandem Mass Spectrometry
Katsuhito HoriKazunobu TsumuraEiichiro FukusakiTakeshi Bamba
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2014 Volume 3 Issue 1 Pages A0033

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Abstract

Supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry was applied to the profiling of sucrose fatty acid esters (SEs). The SFC conditions (column and modifier gradient) were optimized for the effective separation of SEs. In the column test, a silica gel reversed-phase column was selected. Then, the method was used for the detailed characterization of commercial SEs and the successful analysis of SEs containing different fatty acids. The present method allowed for fast and high-resolution separation of monoesters to tetra-esters within a shorter time (15 min) as compared to the conventional high-performance liquid chromatography. The applicability of our method for the analysis of SEs was thus demonstrated.

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© 2014 The Mass Spectrometry Society of Japan
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