布上でのタンパク質直接定量に及ぼす布帛種の影響

抄録

The influence of the weave, thickness, and fiber material of the fabric on the direct determination of the protein in the fabric by the bicinchoninic acid (BCA) method was investigated in this paper. Six kinds of cotton fabrics, with different weaves and thicknesses, and eight kinds of plain-weave fabrics with different fiber materials were used as the test swatches. The K/S value was calculated from the reflectance, based on the color reaction of the BCA method, and the quantitation was evaluated based on the relationship between the K/S value and the protein concentration. Based on the results, it was determined that the influence of the weave and thickness was not significant, and the quantitation was good. Contrastingly, it was found that protein fibers (wool and silk) and the cuprammonium rayon, in which copper ions remained, which react with the C solution of the BCA method, are not suitable for this method.