抄録
A novel method called “lipid–coated ice droplet hydration”for preparing giant vesicles encapsulating hydrophilicmolecules such as enzymes was developed and was utilized to construct a microbioreaction system which mimickedcellular environment. In this article, we first summarize basic concepts of the lipid–coated ice droplet hydrationmethod and then discuss its characteristics for preparing giant vesicles, including abilities of encapsulation ofhydrophilic molecules and control of vesicle size. Subsequently, enzymatic microreaction systems using giant vesi-cles prepared by our method is presented. Enzymatic reactions inside giant vesicles were conducted by transport ofsubstrate molecules via permeation through the lipid membranes of the vesicles. The experimental results demon-strated the possibility of construction of a selective enzymatic reaction system based on membrane permeability ofsubstrate molecules.
