Abstract
Exosomes (30～200 nm in diameter) are small secretory vesicles released from various cell types, and they represent potential next–generation biological tools for delivering therapeutic molecules. In this review, I discuss novel technique for development of intracellular delivery system based on biofunctional peptide–modified exosomes to enhance cytosolic release via a pH–sensitive fusogenic peptide. Additionally, I also discuss importance of active induction of macropinocytosis for internalization of exosomes by cells and provide related delivery technique of exosomes with epidermal growth factor that effectively induces macropinocytosis.
