Abstract
Perfusion culture with cell recycle has been recognized as an effective method to improve the low productivity in animal cell cultivation. Membrane filtration is one of the most effective method for cell recycle, and tangential flow membrane filtration has been used extensively in microbial cultures. In the animal cell cultivation, however, the use of this membrane filtration is still limited, because of the sensitivity of animal cells to hydrodynamic forces. This review paper will introduce the current works for establishment of operating parameters in membrane filtration which maximize permeation flux and minimize membrane fouling while not causing cell damage and lysis.
