膜転写の機構-核酸を中心にして-

抄録

The transfer membrane of high void volume microporous structure has enormously high “specific surface area (surface area/volume)” similar to biomolecules such as nucleic acids, proteins, etc. which are target materials for membrane transfer. Therefore, in order to understand the mechanisms of membrane transfer, scientific investigation of interactions between membrane pore surface and biomolecules should be studied based on colloidal chemistry and surface chemistry.
Characteristics of the transfer membrane are not related to molecular structure of the material itself. However, they are determined by molecular structure of the membrane pore surface.
Basic mechanisms of adsorption on transfer membrane are 1) hydrostatic interaction (ionic bond), 2) hydrophobic interaction (hydrophobic bond), 3) hydrogen bond and 4) van der Waals adsorption.
The transfer solution (0.4 N NaOH) with no salt can be used for the nylon membrane which is tolerant to alkaline condition (pH 11) and is positively charged in that condition. In this case, DNA fragments are completely denatured and transferred to the nylon membrane. Besides the hydrophobic interaction, hydrophilic interaction between membrane pore surface and DNA fragments increases transfer efficiency (yield of DNA).