Preparation and Protein Adsorption Properties of Porous Chitosan Membranes Modified with Acetylsalicylic Acid and Tannic Acid

抄録

Two kinds of affinity membranes were prepared from the porous chitosan membrane (Ch membrane), and studies on adsorption and desorption of a serum protein were made, from the viewpoint that affinity membranes are efficacious against separation and purification of biomaterials. Into the Ch membrane, which was regenerated from chitosan dissolved in aqueous acetic acid by immersing it in an alkaline coagulation bath, acetylsalicylic acid and tannic acid were introduced (Ch-AS and Ch-T membranes). Adsorption of bovine serum albumin (BSA) on the membranes was investigated in a batchwise mode. The Ch membrane showed the strong pH dependence of the adsorption of BSA, whereas the Ch-AS and Ch-T membranes adsorbed BSA in the wide pH region. The amounts of BSA adsorbed on the Ch-AS and Ch-T membranes were smaller than that on the Ch membrane. Recovery of BSA from the modified membranes was also investigated in a dead-end flow mode. High recoveries of BSA from the Ch-AS and Ch-T membranes were obtained, and the concentrations of BSA eluted were higher than those of the feed solutions.