逆ミセルを用いる遺伝子変異の検出
2002 年 27 巻 2 号 p. 89-93
詳細
2002 年 27 巻 2 号 p. 89-93
Reversed micelles, which are nanostructural molecular assemblies, are utilized for the mutation detection of deoxyribonucleic acids (DNAs). The hybridization rate of a complementary single stranded DNA was measured in reversed micelles by detecting the decrease in the absorbance at 260nm with a UV/VIS spectrophotometer. The hybridization rate was significantly reduced when the complementary DNA has a mismatched sequence. The number and the position of the mutation also influenced the hybridization rate in reversed micelles. Therefore, we can readily know the existence of a mutation for a targeted DNA by measuring the hybridization rate. The analysis of sample oligonucleotides (20mer) that relates to the tumor suppressor gene P53 was conducted in the AOT reversed micelle. The hybridization rate was very sensitive to the number and the location of the mismatched sequence in the sample DNAs.
