膜タンパク質の膜間移行現象を利用した人工膜ワクチン調製法の検討
2002 年 27 巻 2 号 p. 94-100
詳細
2002 年 27 巻 2 号 p. 94-100
Membrane protein transfer from influenza virus-infected cells to liposomes was examined in several experimental conditions using gel-filtration, SDS PAGE and fluorescent microscopy. In gel-filtration it was found that membrane protein spontaneously transferred from cell membrane to liposomal membrane during incubation of liposomes with cells, accompanied by some leaks of non-membrane protein.The amount of membrane protein incorporated into liposomal membrane was proportional to that of total protein released from cells. SDS PAGE experiment after immunoprecipitation confirmed the transfer of influenza antigenic protein from influenza virus-infected cell's membrane to liposomal membrane. Fluorescent image was observed all around liposome surface in microscopy using fluorescence antibody, suggesting the transferred antigenic protein was incorporated throughout into liposomal membrane.
