Abstract
Biospecific affinity is retained by immobilizing a molecular recognizable biosubstance on the surfaces of synthetic membranes. A biospecific affinity membrane adsorbs specifically the corresponding molecules to the surface with resulting change in transmembrane potential, which is termed “affinity membrane potential”. The concept of affinity membrane potential is generalized and successfully applied in designing biosensors including immunosensors. Theoretical and experimental aspects of affinity membrane potential is reviewed with current topics on practical use.
