Abstract
Using raw or defatted rice bran as a supplement, 5mg to 20mg per kilogram thiamine was added to the sawdust-based substrates. We examined the mycelial growth, yield and contents of major chemical components and thiamine of the fruit bodies of Lentinula edodes. The addition of thiamine did not influence the mycelial growth, yield or major chemical components of the fruit bodies. However, the fruit bodies developed on the substrate using raw rice bran added with lOmg/kg thiamine contained three times more thiamine than those on the substrates without thiamine, and those on the substrate using defatted rice bran two times. The thiamine contents of the fruit bodies developed on the substrates using raw rice bran declined according to the fruit body flushes during harvest, but those on the substrates using defatted rice bran increased. This meant that the thiamine contents of fruit bodies differed during harvest according to the supplement used for the substrates.
