Abstract
The efficient disruption method for β-1,3-glucan determination from mycelium of Ganoderma lucidum was investigated by employment of mortar, coffee mill and Multi-beads Shocker (MBS). The disruption of mycelium using MBS shaking with metal corn for 90 seconds resulted in the smallest particle size distribution followed the highest extraction efficiency of β-1,3-glucan. Using the mycelium powders disrupted by MBS for 90 sec, the rapid extraction process (the MBS method) including successive use of MBS with glass beads was developed. When an extraction vessel of 2ml was filled up to 35% in volume ratio with extraction mixture, the greatest extraction efficiency was provided. The optimum conditions for the glucan extraction were placing 5 to 15mg of the powdered mycelium in the extraction vessel, and adding the glass beads having 0.5mm diameter, respectively. When this extraction process had been carried out, the total time required for preparations of hot-water extract and cold-alkaline extract was shortened to only 4 hours. Also, the total β-1,3-glucan content in the extract prepared by the new extraction method (the MBS method) was 43% higher than that by the general method reported previously.
