Abstract
The nutritional requirements for the vegetative growth of Sparassis crispa Wulf.: Fr. were investigated with the use of a liquid culture medium. A wide range of carbohydrates served as a carbon source in the medium, which supported growth of S. crispa. Glucose, galactose, mannose, fructose, soluble starch, glycogen and dextrin were especially good carbon sources for mycelial growth. The optimal concentration of the carbon source was 2% in the case of glucose, and when it was more than 3%, the yield of mycelium decreased. The optimal concentration of the carbon source was 5% in case of soluble starch, and mycelium also grew in the concentration of 13%. Yeast extract, Soyton, polypeptone, meat extract and Casamino acid were acceptable nitrogen sources for the growth, while ammonium and nitrate salts were poor nitrogen sources. Alanine, valine, aspartic acid, glutamic acid, arginine, lysine and serine had the effect on the vegetative growth, but there was nothing to match with Casamino acid. The optimal concentration of the nitrogen source in the medium was 0.01-0.04%N.
