Abstract
Metabolic activation of promutagens in the Ames test was performed using a crude enzyme solution (S9) extracted from a rat liver. We hope to be able to substitute enzymes produced by microorganisms for enzymes extracted from animals. We tried metabolically activating promutagens using an extracellular and mtracellular enzyme solution prepared from white-rot fungi. The mtracellular enzyme solution from Corlolus versicolor reacted strongly to 2-Ammoanthracene (2AA) and 3-amino-1-methyl-5H-pyndo[4,3-a]-indole (Trp-P-2) in comparison to enzyme solutions from Phanerochaete chrysosporium and extracellular enzymes from C. versicolor. We examined the various kinds of promutagens with the mtracellular enzymes of C. versicolor. Assuming the number of revertants to be 1.0 when mtracellular enzymes are not added, it was found that the number of revertants increased 2.8 times in Aflatoxin B_1 (AFB_1), 3.1 times in 2-Aminofluorene (2AF), 3.6 times in 4-Nitroquinoline (4NQO), 3.7 times in Benzo[a]pyrene (BaP), 4.0 times in 2AA and 4.7 times in Trp-P-2. These results indicate that the crude enzymes of the white-rot fungi perform the metabolic activation of promutagens.
