Abstract
Vitamin B_1 contents of Lentinula edodes fruit bodies cultivated on substrates with vitamin B_1 hydrochloride using two techniques were examined in commercial production facilities. One is a standard technique in which cultivation bags are removed and the other is a new technique in which water is poured into between bags and substrates. In both cases, vitamin B_1 content in the fruit bodies grown on substrate containing vitamin B_1 hydrochloride were greater than those grown on substrates without. Similarly, vitamin B_1 content in Pleurotus ostreatus and Grifola frondosa fruit bodies cultivated on substrates containing vitamin B_1 hydrochloride were also higher than in fruit bodies grown on substrates without. Vitamin B_1 hydrochloride contents in pilei at the dry basis ofL. edodes and P. ostreatus fruit bodies grown on substrates with or without were greater than the contents in stipes.