Abstract
Laccase production by Strobilurus ohshimae and its use for decolorizing phenolic dyes were studied. S. ohshimae strain Nagaoka 1-1 was cultivated for 30 days on sugi wood meal medium (sugi wood meal : rice bran : water = 10:3:30). Laccase activity was 405U/g of medium (dry weight), which is higher than previously reported levels. Decolorization of 7 phenolic dyes was then tested using this enzyme, and all were decolorized. Cresol red was decolorized most, by more than 40% over 2 hours. The addition of 1-hydroxybenzotriazole (HOBt), which is redox mediator, increased rate of decolorization. With addition of HOBt, cresol red was decolorized completely in 40 min, and remazole brilliant blue R in 80 min.
