2011 Volume 19 Issue 2 Pages 93-99
The effects of the addition of hydrolyzates containing a lot of low-molecular weight hyaruronic acid (LMW-HA) from comb (chicken) to the culture medium was tested on three commercial strains of Lentinula edodes and Flammulina populicola NBRC 7777 for vegetative mycelial growth and fruit-body yield. The dry weights of mycelia from L. edodes were increased by about 1.75 times (Mori 465 strain), 1.44 times (Meiji Strain) and 1.67 times (Kinko 324 strain) with the addition of comb hydrolyzates (0.125%), respectively. Also, mycelial dry weights and fruit-body yields of F. populicola NBRC 7777 were increased by about 1.70 times and 1.75 times that of the control, respectively. To elucidate the taking of LMW-HA from comb hydrolyzates in cell, N-acetyl-D-glucosamine and glycosaminoglycan contents in mycelia and fruit-bodies of F. populicola were analyzed. N-Acetyl-D-glucosamine contents were increased by about 2.85 times (vegetative mycelia) and 2.69 times (fruit-bodies) with the addition of LMW-HA from comb hydrolyzates in the medium. On the other hand, the concentration of glucosaminoglycan in fruit-bodies was from 0.52% (69.30μg/Petri dish) to 0.35% (469.0μg/Petri dish) even though glucosaminoglycan was not detected in the control culture (without the addition of comb hydrolyzate) when the hydrolyzate (0.05-0.5%) containing LMW-HA was added to the culture medium.
