Abstract
In order to reveal the regulation of the glucoamylase gene from Pholiota microspora, the promoter region about 2734 bp upstream of the start codon of PnGlu1 gene was amplified and characterized. Two TATA box like sequences and consensus sequences of two yeast mating factor alpha1 (MATα1) and three mating factor a1 (MATa1) binding sites were discovered. To investigate the relationship between regulation of PnGlu1 and the mating type system, PnGlu1 gene expression was investigated in a monokaryon, a dikaryon and four transformants into which were introduced homeodomain protein genes. PnGlu1 gene expression was induced in the dikaryon, and PnGlu1 gene expression in four transformants was higher than that in the monokaryon. To investigate the relationship between regulation of PnGlu1 and fruiting body development, P. microspora was cultivated in sawdust media and then quantitative reverse transcription-PCR was carried out. The PnGlu1 expression level, glucoamylase activity and glucose content dramatically increased until fruiting. These results suggested that glucoamylase gene expression is closely related with dikaryotization and fruiting body development.
