Abstract
Mucidula venosolamellata is an edible mushroom in Japan. In this study, we investigated culture characteristics during basidiospore germination, subsequent colony formation, and dikaryotic hyphae formation. The macromorphological features of the fruiting bodies were also examined. When basidiospores of this mushroom were spread on malt extract agar plates at 25℃, basidiospore germination began within 4 h, and all the basidiospores germinated after 22 h of incubation. When a basidiospore isolate was crossed with a compatible basidiospore isolate, F1 dikaryotic mycelium bearing clamp connections was observed in the contact zone at 5 days after incubation. The resulting dikaryotic mycelia were cultured on sawdust medium for 30 days and were then transferred to a cool room at 15℃ with 85% relative humidity. Fruiting bodies formed within 30 days after the transfer. The fruiting bodies of the F1 dikaryotic strain were bigger than those of the parental strains. These results imply that useful strains could be created by cross-breeding between particular monokaryotic isolates that possess macromorphologically different characteristics.
