Abstract
In this study, we identified five manganese peroxidase genes (PnMnPs) in the Pholiota microspora haploid genome. Their amino acid sequences showed high similarity and were used to construct a phylogenetic tree. PnMnP5, 3, 2 and 4 were clustered tightly, but PnMnP1 was clustered relatively far from PnMnP5. qRT-PCR showed that PnMnP5 was the only MnP gene that was strongly transcribed, showing 15-fold higher expression than other PnMnPs in M4 liquid medium, while transcription of PnMnP5 in sawdust medium was 100 times higher than in M4 liquid medium. These results indicate that PnMnP5 plays a major role in the ligninolytic peroxidase reaction during mycelial growth in P. microspora. Based on a comparison of the position of introns, the phylogenetic relationships among PnMnPs and the predominant expression of PnMnP5, we believe that all PnMnPs are of the same origin and that they were amplified by duplication events in the ancient P. microspora genome.
